Plasmid

Part:BBa_K5218004:Design

Designed by: Rui Zhang, Lichuan Chen, Xiaojuan Wang   Group: iGEM24_BGI-MammothEdu-South   (2024-08-18)


pS1300-RcTAL-GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 490
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 490
    Illegal AgeI site found at 646
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the RcTAL is a gene from bacteria, before synthesising the RcTAL CDS, we did codon optimisation to make it more compatible to the the plant system.


Source

RcTAL CDS sequence was synthesised, and the pS1300-GFP plasmid backbone was obtained from iGEM BGI-MammothEdu-China team (2023)

References

1. Arjun Khakhar, Colby G Starker, James C Chamness, Nayoung Lee, Sydney Stokke, Cecily Wang, Ryan Swanson, Furva Rizvi, Takato Imaizumi, Daniel F Voytas (2020) Building customizable auto-luminescent luciferase-based reporters in plants. eLife 9:e52786